Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [2]
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Validation data
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- Product number
- NBP2-34728-0.1 mg - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal TNF-alpha Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production.
- Reactivity
- Human, Mouse, Rat, Canine, Feline, Rabbit, Zebrafish
- Host
- Mouse
- Isotype
- IgM
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TNF-alpha Antibody (TNF706) - Azide and BSA Free [NBP2-34728] - Formalin-fixed, paraffin-embedded human Erdheim Chester disease (also known as polyostotic scsclerosing histiocytosis) stained with TNF alpha Monoclonal Antibody (TNF706).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TNF-alpha Antibody (TNF706) - Azide and BSA Free [NBP2-34728] - Formalin-fixed, paraffin-embedded Rat Pancreas stained with TNF-alpha Antibody (TNF706)